Composite

Part:BBa_K322312

Designed by: Maria Kowal   Group: iGEM10_Edinburgh   (2010-09-25)


artificial operon assembled from luxCD and luxE

lux CDE codes for a fatty acid reductase complex that makes the fatty acids， which is necessary for the luciferase mechanism. Among them, LuxC codes for the enzyme acyl-reductase and lux D codes for acyl-transferase, and lux E makes the proteins needed for the enzyme acyl-protein synthetase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1332


[edit]
Categories
Parameters
None